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To analyze the constraints imposed by Lon about the DHFR mutational landscape in structural detail, we outlined a ∆selection coefficient for every amino acid residue at Every place as the difference between the +Lon and –Lon selections (determine 3A). The ∆assortment coefficient values have been most adverse at positions inside the effective classification and at positions with a native VILMWF or Y amino acid residue (determine 3B, excludes Intolerant positions from –Lon collection); Total, mutations at positions with native hydrophobic residues are enriched for negative ∆range coefficients (determine three—determine dietary supplement 1A).
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